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Bruker Daltonics
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In-depth Characterization of Neutral and Acidic Glycopeptides by ZIC-HILIC Enrichment and Mass Spectrometry

Type: Application

Poster

Expression Proteomics
Number: Technology

DGMS 2010

Ion Trap, MALDI-TOF
Year Products

2010

EASY-nLC, ultrafleXtreme, amaZon ETD
  Author
 

Jessica Wohlgemuth (1), Sven Andrecht (1), Andrea Schneider (2), Arndt Asperger (2), Franz-Mayer-Posner (2), Anja Resemann (2), (1) Merck KGaA, Darmstadt; Germany (2) Bruker Daltonik GmbH, Bremen; Germany
  Reference
 

DGMS
 

Abstract
 

  • A reproducible glycopeptide enrichment method is described
  • ESI-Ion-Trap-MS profiles allowed to monitor the reproducibility of the enrichment
  • MALDI-MS/MS spectra of glycopeptides allowed to
  • ID the aglycone
  • Localize the N-glycosylation site
  • ID the glycan
 

Related Products
 

DGMS 2010
 

Download

 

DGMS2010_198__Poster_Merck_Glycoproteins_HIPIC_enrichment_sialic_acid.pdf
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