N- and C-terminal Protein Sequencing Using in-source-decay (ISD) MALDI MS
| Type: | Application |
Application Note |
Expression Proteomics |
| Number: | Technology |
2000902 |
MALDI-TOF |
| Year | Products |
2010 |
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| Author | |
Ejvind Mortz, Thanh Ha Nguyen, and Janne Crawford. Alphalyse A/S, Unsbjergvej 4, Odense, Denmark. Email correspondence: mortz @ alphalyse.com Alphalyse A/S Unsbjergvej 4 Dk-5220 Odense sø, Denmark Tel +45 6310 6500 |
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| Reference | |
Alphalyse Application Note # 2000902 |
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Abstract |
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Analytical characterization of purified proteins, in particular recombinant proteins for drug discovery and development, requires confirmation of the full length protein sequence. Here we present a powerful mass spectrometry method (MALDI ISD) that provides partial sequence information of the intact protein with up to 20-50 amino acid residues from both the N-terminal and C-terminal in one single analysis. The analysis can thus confirm expression and purification of the full length protein sequence, and detect unexpected truncations and modifications of the termini. The MALDI ISD analysis can also be applied to N-terminally blocked and PEGylated proteins. Alphalyse provides protein analysis services and here we present results obtained on a variety of proteins.
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