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Differentially Expressed Proteins Detected on Chromatographic Retentive Surface Capture by Mass Spectrometry
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
ASMS 2010 |
MALDI-TOF |
| Year | Products |
2010 |
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| Author | |
Amanda Bulman(1), Enrique Dalmasso(1), Steve Roth(1), Fiona Plows(1), Detlev Suckau(2) and Martin Schuerenberg(2); 1Bio-Rad Laboratories, Inc., Hercules, CA, USA; 2Bruker Daltonics, Bremen, Germany |
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| Reference | |
ASMS 2010 |
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Abstract |
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The effects of chromatographic enrichment on both sensitivity and selectivity of protein detection have been long established for linear TOF MS top-down biomarker profiling. In this study we utilize various chromatographic surface functionalities to demonstrate differential expression of native peptides and proteins in beer yeast lysates, prepared after incubation with and without three different sweeteners. Profiling utilized the Lucid Proteomics System™, a combination of ProteinChip arrays with high-resolution MALDI-TOF/TOF mass spectrometry. Direct MS/MS sequence analysis of peptides from chromatographic arrays, with and without small scale chromatographic enrichment, was performed to identify peptides of interest. |
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