In-depth Characterization of Neutral and Acidic Glycopeptides by ZIC-HILIC Enrichment and Mass Spectrometry
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
ASMS 2010, TP06-163 |
MALDI-TOF, Ion Trap |
| Year | Products |
2010 |
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| Author | |
Jessica Wohlgemuth 1, Sven Andrecht 1, Andrea Schneider 2, Anja Resemann 2, Arndt Asperger 2, Ralf Schaefer 2, (1) Merck KGaA, Darmstadt, Germany; (2) Bruker Daltonik Bremen, Germany |
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| Reference | |
ASMS |
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Abstract |
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• A reproducible glycopeptide enrichment method is described • ESI-Ion-Trap-MS profiles allowed to monitor the reproducibility of the enrichment • MALDI-MS/MS spectra of glycopeptides allowed to - ID the aglycone- Localize the N-glycosylation site- ID the glycan |
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