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Advances in Molecular Histology with the MALDI Molecular Imager
| Type: | Application |
Application Note |
Expression Proteomics, Clinical Proteomics |
| Number: | Technology |
MT-89 |
MALDI-TOF |
| Year | Products |
2007 |
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| Author | |
Sören-Oliver Deininger1, René Krieg1, Sandra Rauser2, Axel Walch2 [1] Bruker Daltonik GmbH, Bremen, Germany [2] Institute of Pathology, GSF Neuherberg, Germany |
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| Reference | |
Application Note # MT-89 |
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Abstract |
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As MALDI molecular imaging technology attracts increasing attention in the field of histopathology, obviously one feature is most crucial to the interpretation of the MALDI images: Their unambiguous correlation with histology. First attempts involved either histological staining of the tissue sample with a MALDI compatible dye followed by MALDI imaging analysis or histological staining and MALDI imaging of consecutive sections. Both approaches have limitations: The conclusions obtained from MALDI compatible stains are limited and the histological details observed on consecutive sections are impossible to match at the 20 - 50 μm level. However, a new approach provides a significant step towards molecular histologyTM: „Post acquisition staining“, i. e. the staining of the sample after the MALDI spectra acquisition on the same tissue section [1,2]. This approach allows the full scale interpretation of the MALDI imaging result both on the molecular and on the histological level with unambiguous correlation on the 20 - 50 μm level. |
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