Accuracy of the API Campy System, the Vitek 2 Neisseria-Haemophilus (NH) Card and the Matrix-Assisted Laser Desorption/Ionisation Time-of-flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Campylobacter and Related Organisms
| Type: | Application |
Scientific Paper |
Clinical Proteomics |
| Number: | Technology |
10.1111/j.1469-0691.2010.03328.x |
MALDI-TOF |
| Year | Products |
2010 |
Microflex LT, MALDI Biotyper |
| Author | |
Martiny D, Dediste A, Debruyne L, Vlaes L, Ben Haddou N, Vandamme P, Vandenberg O |
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| Reference | |
Clinical Microbiology and Infection |
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Abstract |
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Biochemical identification of Campylobacter and related organisms is not always specific and may lead to diagnostic errors. The API Campy, the Vitek 2 system and Matrix-assisted desorption/ionisation time-offlight mass spectrometry (MALDI-TOF MS) are commercially available methods that are routinely used for the identification of these microorganisms. In the present study, we used 224 clinical isolates and 10 reference strains previously identified by multiple PCR assays, whole-cell protein profiling and either DNA-DNA hybridisation or sequencing analysis to compare the reliability of these three methods for the identification of Campylobacter and related pathogens. The API Campy accurately identified 73.8% of Campylobacter coli and 94.4% Campylobacter jejuni subsp. jejuni, but it failed to correctly identify 52.3% of other Epsilobacteria. The Vitek 2 NH card correctly identified most C. jejuni subsp. jejuni (89.6%) and C. coli (87.7%) strains, which account for the majority of campylobacteriosis reported in humans, but it failed in the identification of all of the other species. Despite a good identification rate for both C. jejuni subsp. jejuni and C. coli, both methods showed poor sensitivity in the identification of related organisms, and additional tests were frequently needed. Contrary to API Campy and Vitek, MALDI-TOF MS correctly identified 100% of C.coli and C.jejuni tested. With an overall sensitivity of 98.3% and a short response time, this technology appears to be a reliable and promising method for the routine identification of Campylobacter and other Epsilobacteria.
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