Biomarker Discovery and Identification by MALDI Molecular Tissue Imaging and Top-down Sequencing

Application Note

Expression Proteomics

MT-108/LCMS-59

MALDI-TOF, Ion Trap

2011

amaZon ETD ultraflex TOF/TOF BioTools flexImaging PRIME

Markus Meyer, Sandra Rauser, Axel Walch, Arnd Ingendoh, Detlev Suckau

MT-108/LCMS-59, #282530

Abstract

There is an urgent need for new and better biomarkers
for use in cancer diagnosis and therapy decision-making
and for delivering new insights into biological processes.
Conventional proteomics approaches have addressed
this need for quite some time. MALDI molecular
tissue imaging has recently emerged as a fully mature
proteomics tool for the direct determination of protein
expression in tissue. This study presents an integrated
workflow – based on Bruker’s PRIME (Proteomics through
Integrated MALDI and ESI) solution – for biomarker
discovery and identification in a complete Top-down
proteomic strategy. The strategy begins with tissue
screening by MALDI imaging, and is using statistical tools
to flag discriminatory proteins. Proteins are identified by
ETD/PTR analysis in the amaZon ion trap. The breast
cancer study presented here reveals a novel putative
biomarker which may become relevant for therapeutic
decision-making.

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