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Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpoB gene sequencing for species identification of bloodstream infection staphylococcal isol
| Type: | Application |
Journal Article |
Expression Proteomics, Clinical Proteomics |
| Number: | Technology |
10.1111/j.1469-0691.2010.03181.x |
MALDI-TOF |
| Year | Products |
2010 |
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| Author | |
Spanu T, De Carolis E, Fiori B, Sanguinetti M, D'Inzeo T, Fadda G, Posteraro B. |
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| Reference | |
Clin Microbiol Infect |
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Abstract |
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Abstract As a result of variable expression of biochemical characters, misidentification by conventional phenotypic means often occurs with clinical isolates belonging to the Staphylococcus species. Therefore, we evaluated the use of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) for identification of 450 blood isolates from most relevant staphylococcal species, using sequence analysis of the rpoB gene as the reference method. A correct species identification was obtained in 99.3% (447/450), with only three isolates being misidentified. In addition, MALDI-TOF correctly identified all the staphylococcal subspecies studied, including S. capitis subsp. capitis and subsp. urealyticus, S. cohnii subsp. urealyticus, S. hominis subsp. novobiosepticus and subsp. hominis, S. saprophyticus subsp. saprophyticus, S. schleiferi subsp. schleiferi and S. sciuri subsp. sciuri. Thus, MALDI-TOF MS-based species identification of staphylococci can be routinely achieved without any substantial costs for consumables, also instead of that based on effective but labour-intensive DNA sequence analysis.
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