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Expanded Collisional Energy Files for Automated Top Down and Bottom up Analysis on solariX
| Type: | Application |
Application Note |
Expression Proteomics |
| Number: | Technology |
FTMS-40 |
FTMS |
| Year | Products |
2010 |
solariX |
| Author | |
Aaron Behr, Jeremy Wolff, Christopher Thompson The Rivers School, Weston MA USA Bruker Daltonics, Inc., Billerica MA USA |
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| Reference | |
FTMS-40, #272669 |
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Abstract |
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Collisionally Induced Dissociation (qCID) energies are optimized on solariX for both peptide and protein species. To avoid over estimating the energies, only assignable fragment ions (b- and y-type) are included within the efficiency calculations. A single collisional energy file (cef) is created for LC-MS/MS applications that can be easily expanded via the procedures outlined within.
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