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Human cell-line phosphoproteome mapping using a dual-funnel ETD ion trap

Type: Application

Poster

Expression Proteomics
Number: Technology

HUPO 2011 #P1643

GC/MS, Ion Trap
Year Products

2011

  Author
 

Chia-fengTsai1, Pei-Yi1, Stephanie Kaspar2, Andrea Kiehne2, Pierre-Olivier Schmit3, Yu-JuChen1 1Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, Republic of China 2Bruker Daltonik GmbH, Bremen, Germany 3Bruker DaltoniqueS.A., Wissembourg, France
  Reference
 

HUPO 2011 #P1643
 

Abstract
 

• The increase in sensitivity and duty cycle resulting from the dual funnel architecture makes it possible to see the complete sample content from a single run
• Routine and robust combined CID and ETD experiments yield complementary results which enable to map a phosphorylationsite in most cases
• The combination of an efficient phosphopeptideenrichment process with this alternated experiment has proven to be an excellent routine tool to be used in ourphosphorylationsites mapping studies
 

Related Products
 

HUPO2011
HUPO 2011
 

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Human_cell-line_phosphoproteome_mapping_using_a_dual-funnel_ETD_ion_trap_P1643.pdf
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