ICPL Labeling in Functional Proteomics Experiments: Substrate Identification of the Extracellular Protease ADAMTS1 using SDS-PAGE LC-MS/MS

Application Note

Expression Proteomics

LCMS-56

Ion Trap

2010

Francesc Canals1, Nuria Colomé1, Joan J. Bech-Serra1, Juan Carlos Rodríguez-Manzaneque2 1 Vall d’Hebron Institute of Oncology (VHIO), Vall d’Hebron University Hospital, Barcelona 2 Pfizer-University of Granada-Andalusian Government Center for Genomics and Oncological Research (GENYO), Granada Wolfgang Jabs, Markus Lubeck, Carsten Baessmann, Detlev Suckau Bruker Daltonik GmbH, Bremen

Application Note # LCMS-56

Abstract

Isotope-Coded Protein Label (ICPL [1,2]) is known as
an accurate protein labeling strategy for quantitative
proteomics which enables protein pre-fractionation and
therefore provides reduction of complexity, detection
of protein isoforms, posttranslational modifications,
and investigations of enzymatic reactions. Such an
investigation is demonstrated in a workflow using SDS-PAGE
for protein pre-fractionation, a Bruker Ion Trap mass
spectrometer, and ProteinScape^TM for quantitative analysis.

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