Mining the Human Placenta Proteome > 2000 Proteins deep using CID/ETD on an Ion Trap

Poster

Expression Proteomics

HUPO 2009 A156

Ion Trap

2009

HUPO 2009 A156

Abstract

•The amazon ion trap delivers up to 5,000 resolution at 8,100 amu/sec scan speed, 0.2 amu mass accuracy and attomol sensitivity for protein ID from complex proteomes.

•~2,200 proteins were identified using a 5-tier approach, the largest set of protein ID from human placenta to date.

•CID identifies more proteins than ETD which adds 20% extra proteins compared to CID alone.

•Gas phase fractionation increases protein ID by a very modest ~10% compared to full scan analysis, but it mostly improves sequence coverage.

•The combination of CID, ETD and gas phase fractionation doubles the number of identified phosphorylation sites. 

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HUPO 2009

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