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Bruker Daltonics
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Spatially Resolved Tryptic Digest on Tissue using the ImagePrep

Type: Application

Application Note

Number: Technology

MT-98

MALDI-TOF
Year Products

2009

ultraflex III TOF/TOF, ImagePrep
  Author
 

Martin Schürenberg, Sören Deininger, Michael Becker; Bruker Daltonics, Bremen, Germany
  Reference
 

Bruker Daltonics Application Note MT-98
 

Abstract
 

Top-down capability is a major advantage of MALD imaging, allowing the analysis of intact endogenous peptides and proteins. However, performing a tryptic digest directly on
the tissue can be beneficial for several reasons:

  • Proteins too large for the mass range of a typical MALDI imaging experiment can be digested with trypsin and the resulting peptides analyzed.
  • Fragmentation and identification of tryptic peptides is easier than for endogenous peptides.
  • Tryptic digestion is the method of choice for the analysis of formalin-fixed paraffin embedded (FFPE) tissue.

To be compatible with MALDI imaging, the localization of peptides generated by a tryptic digest procedure must match with the original localization of the undigested and diffusion across the tissue must be prevented. In addition, compatibility with subsequent histological staining is important, especially for the analysis of tumor sections. The ImagePrep, with its ability to deposit minute droplets onto tissue in a controlled environment is well suited to perform enzymatic digests on tissue. Here we describe the use of the ImagePrep for this purpose.
 

Related Products
 

ImagePrep
Tissue Imaging
 

Download

 

MT-98_ebook.pdf
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