High Performance TLC-MALDI

Type: Application

Poster

Expression Proteomics

Number: Technology

PMM: 386

MALDI-TOF

Year Products

2009

  Author
 

Martin Schürenberg1, Beate Fuchs2, Annabell Bischoff2, Rosemarie Süß2, Detlev Suckau2, Jürgen Schiller2 and Gerda Morlock3

  Reference
 

Poster IMSC 2009

 

Abstract

 

HPTLC-MALDI is a largely automated, software-supported new method:

  • Chromatographic resolution of HPTLC is maintained throughout the MALDI analysis Fig. 1
  • Reproducibility of HPTLC-MALDI is in the 10 % range Fig. 2
  • Sensitivity of HPTLC-MALDI for lipids is in the 100 ng range Fig. 3
  • Structural analysis can directly follow up using HPTLC-MALDI-MS/MS Fig. 4
  • Suitable for lipid analysis for clinical, nutritional, pharmaceutical and cosmetic analysis

 

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