Challenges in the investigation of the metabolic changes in Nicotiana attenuataduring insect herbivory using an improved HPLC-TOF-MS method
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Poster |
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Technology |
ENv34-Tu |
OTOF |
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2009 |
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Author |
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Matthias Schöttner1; Eva Rothe1; Emmanuel Gaquerel1; Beatrice Berger1; Jörg Sauer2; Birgit Schneider2; Gabriela Zurek2; Aiko Barsch2 and Ian T. Baldwin1 |
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Reference |
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Abstract |
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- By means of internal standards and a pooled quality control sample, the reproducibility of the analytical method was monitored.
- PCA and t-test were successfully applied to filter out metabolic changes.
- The identification of regulated metabolites can be achieved based on molecular formulae derived from accurate mass spectra plus chemical knowledge.
- The same procedure will be applied to investigate the influence of the genetic modification.
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