GC/APCI-TOF MS: a new valuable tool for analysis of biofluids in metabolomics studies

Poster

Metabolomics Studies

DGMS 2010

UHR-TOF

2010

maXis

Tiziana Pacchiarotta1, Alegría Carrasco-Pancorbo1, Ekaterina Nevedomskaya1, Marina Behrens2, Thomas Arthen-Engeland2, Gabriela Zurek2, Jacobus J. van Hilten (3), Andre M. Deelder (1), Oleg A. Mayboroda (1); (1) LUMC, Biomolecular Mass Spectrometry Unit, Dept. of Parasitology, Leiden, NL; (2) Bruker Daltonik GmbH, Bremen, D; (3) LUMC, Dept. of Neurology, Leiden, NL

DGMS

Abstract

• The derivatization reaction is a crucial step in GC-MS analysis. It is mandatory to optimize its conditions in depth as it can be themajor source of variation in complex matrices.
• During GC/APCI ionization of TMS derivatives the predominant ion observed is [M+H]+.
• The comparison of APCI and EI spectra reveals similarities and differences concerning presence/absence of the (pseudo) molecular ion and key fragments.
• The combination of GC/APCI with UHR-TOF MS directly enables the assignment of molecular formulae to the TMS derivatives and its fragments. This significantly facilitates spectra interpretation.

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