High Performance TLC-MALDI

Poster

Expression Proteomics

DGMS 2010

MALDI-TOF

2010

ultrafleXtreme, TLC-MALDI software, TLC-MALDI adapter

Martin Schürenberg(1), Beate Fuchs (2), Annabell Bischoff (2), Rosemarie Süß (2), Detlev Suckau (1), Jürgen Schiller (2), Gerda Morlock (3) and Ulrike Anders (1) (1) Bruker Daltonik GmbH, Germany; (2) University of Leipzig, Germany; (3) University of Hohenheim, Stuttgart, Germany

DGMS

Abstract

HPTLC-MALDIis a largely automated, software-supported new method:

• Chromatographic resolution of HPTLC is maintained throughout the MALDI analysis Fig. 1
• Reproducibility of HPTLC-MALDI is in the 10 % range Fig. 2
• Sensitivity of HPTLC-MALDI for lipids is in the 100 ngrange Fig. 3
• Structural analysis can directly follow up using HPTLC-MALDI-MS/MS Fig. 4
• suitable for lipid analysis for clinical, nutritional, pharmaceutical and cosmetic analysis

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