ImmunoImaging: Investigation of Tissue-less Tissue Imaging and Simplification of the Tissue Proteome Using Animal Models
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
ASMS 2010 |
MALDI-TOF |
| Year | Products |
2010 |
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| Author | |
Fiona Plows(1); Mariana Rusa(1); Amanda Bulman(1); Steve Roth(1); Enrique Dalmasso(1); Matt Hammond(1); Martin Schuerenberg(2); Detlev Suckau(2); 1Bio-Rad Laboratories, Inc., Hercules, CA; 2Bruker Daltonics, Bremen, Germany |
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| Reference | |
ASMS 2010 |
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Abstract |
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IntroductionCrude tissue imaging is the experimental equivalent of serum, with its 15 orders of magnitude of concentration, being restricted to neat investigation only, with the additional complication that the sample is inherently heterogeneous. In serum, it is straightforward to quantify a certain protein of interest using ELISAs, but specific detection by an untargeted method such as LC-MS may fail. Affinity based enrichment is a useful method for targeted analysis as it enriches the molecule of interest significantly and permits mass spectrometric detection even without further chromatographic workup. |
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