Isotope-Coded Protein Labeling (ICPL) for Quantitative Proteomics Workflows Compatible with Protein Separation Strategies
| Type: |
Application |
Application Note |
Expression Proteomics |
| Number: |
Technology |
MT-106 |
MALDI-TOF |
| Year |
Products |
2010 |
ultrafleXtreme, EASY-nLC, ProteinScape, PROTEINEER sp, SERVA ICPL Quadruplex PLUS Kit (#258968) |
| |
Author |
| |
Anja Resemann, Waltraud Evers, Detlev Suckau;
Bruker Daltonik GmbH, Bremen, Germany |
| |
Reference |
| |
Bruker Daltonics Application nOte MT-106 |
| |
Abstract |
| |
The new Serva ICPLTM Quadruplex PLUS (ICPL 4-plex)
protein quantification kit was used to establish instrument
and data analysis methods on the ultrafleXtreme MALDITOF/
TOF instrument. Resulting data were analyzed
using ProteinScape 2.1 that provide good quantification
results from ICPL 4-plex measurements. We analysed a
protein mixture that was labelled in 4 states with defined
quantities to validate the compatibility of the ICPL 4-plex
Kit with the bioinformatics suite ProteinScape 2.1, and the
MALDI-TOF/TOF.
|
| |
Related Products |
| |
ultrafleXtreme series |
| |
Download |
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