Time-course Reproducibility of Clinical Samples using Retentate Chromatography MALDI-TOF Mass Spectrometry
| Type: | Application |
Poster |
Expression Proteomics, Clinical Proteomics |
| Number: | Technology |
ASMS 2010, ThP05 129 |
MALDI-TOF |
| Year | Products |
| Author | |
Sabine Jourdain1, Amanda Bulman2, Waltrau Passlack3, Sonia Hartwig3, Stefan Lehr3, Steve Roth2, Fiona Plows2, and Enrique Dalmasso21Bio-Rad Laboratories, Division Bio-Recherche, 92430 Marnes la Coquette, France; 2Bio-Rad Laboratories, Inc. Hercules, CA; 3Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Dusseldorf, Germany |
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| Reference | |
Abstract |
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Good study design and implementation of standard operating procedures ensure the level of experimental reproducibility that is required to achieve successful clinical biomarker discovery.In this study organized over 5 non-consecutive days, monitoring variability using a reference sample and the statistical tools provided in the Lucid Proteomics Software were imperative. Very acceptable intra-assay and day-to-day CVs of 10-14 % were demonstratedand no bias was detected that could compromise the discovery of scientifically sound candidate markers.
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