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Accurate Quantification with ICPL: The Quantification Study of the ABRF Proteomics Research Group 2006
| Type: | Application |
Application Note |
Expression Proteomics |
| Number: | Technology |
MT-83 |
MALDI-TOF |
| Year | Products |
2006 |
ultraflex II TOF/TOF, ICPL, PAC, PROTEINEER-LC, PROTEINEER fc |
| Author | |
| Reference | |
Abstract |
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The objective of the ABRF PRG study was to quantify 8 proteins in 2 mixtures. An ultraflex IITM was used to analyze ICPLlabeled protein digests using an LC-MALDI approach under control of the WARP-LCTM software. An average quantification error of 9.3 % was achieved using high performance instrumentation, Prespotted AnchorChips, ICPL technology and sophisticated bioinformatics tools - being the best result amongst all iTRAQTM and ICPLTM data returned to the study. |
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