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Analysis of Alzheimer’s Disease Markers in Complex Biological Samples using Derivatized Arrays
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
ASMS 2010 |
MALDI-TOF |
| Year | Products |
2010 |
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| Author | |
Mariana Rusa(1), Steve Roth(1), Amanda Bulman(1), Enrique Dalmasso(1), Matt Hammond(1), Fiona Plows(1) and Martin Schuerenberg(2); 1Bio-Rad Laboratories, Inc, Hercules, CA, USA; 2Bruker Daltonics, Bremen, Germany |
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| Reference | |
ASMS 2010 |
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Abstract |
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IntroductionThe use of derizatived arrays and time-of-flight mass spectrometry for the top-down profiling of intact proteins has previously yielded a large number of candidate biomarkers, biological indicators of a phenotypically altered state, from complex biological samples. We show here that this combination is also of value for the targeted assay of established biomarkers such as amyloid beta peptides. Amyloid beta peptides can be difficult to study using standard methods due to their multiplicity and small size. Numerous fragments potentially contained in samples creates issues of cross-reactivity and incomplete results with traditional methods such as ELISA. Detection by mass spectrometry has the advantage of enabling the clear distinction between fragments that may share the same antibody recognition sequence. Multiple amyloid beta peptides can be captured simultaneously from biological samples using a targeted proteomics approach combining immunological sample enrichment and MALDI: Mass Spectrometry In-Situ ImmunoAssay (MaS-ISIA). |
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