| Type: |
Application |
Technical Note |
Expression Proteomics, Clinical Proteomics |
| Number: |
Technology |
TN-41 |
MALDI-TOF |
| Year |
Products |
2011 |
|
| |
Author |
| |
Arndt Asperger, Martin Schürenberg,
Detlev Suckau, Marcus Macht
Bruker Daltonik, Bremen, Germany |
| |
Reference |
| |
Bruker Daltonics Technical Note TN-41, #274733 |
| |
Abstract |
| |
A new MALDI target with 1536 spots per plate is
described. The new AnchorChipTM target provides
substantial improvements to the LC-MALDI analysis of
complex biological samples. The MTP AnchorChip 1536 TF
can accommodate fractions collected from an extended LC
gradient (up to four hours) or, alternatively, from multiple
shorter LC gradients spotted in sequence. Complete
MALDI-TOF/TOF analysis of all the spotted fractions can
be easily achieved within a single run without the need of
target exchange. This provides significant improvements
regarding utilization of the high throughput capacity offered
by high speed MALDI-TOF/TOF systems such as Bruker´s
ultrafleXtremeTM. Applying expanded LC gradients to the
LC-MALDI analysis of complex proteomics samples leads to
an improved depth of analysis resulting in a higher number
of protein IDs and peptide assignments. In the benchmark
example shown here, a single injection of 500 ng E.coli cell
lysate digested with trypsin yielded more than 900 protein
IDs based on more than 6000 assigned peptides.
|
| |
Related Products |
| |
ultrafleXtreme series
autoflex speed series |
| |
Download |
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