TN-25 Simple and Fast Two-Dimensional LC-MS on the Bruker EASY-nLC: EASY-2D

Technical Note

Expression Proteomics

TN-25

Ion Trap, FTMS, OTOF

2008

Andrea Kiehne1, Marina Behrens1, Thomas Hagedorn1, Morten Beck Trelle2, Michael Barrett Andersen2 [1] Bruker Daltonik, Bremen, Germany [2] Proxeon, Odense, Denmark

Bruker Daltonics Technical Note TN-25

Abstract

Analysis of complex biological protein/peptide samples
by tandem mass spectrometry (MS/MS) requires
extensive fractionation of the sample. Automated twodimensional
liquid chromatography (2D-LC) is a promising
separation technology which, coupled on-line with MS/
MS instrumentation, shows great analytical potential
in the field of proteomics. 2D-LC is often based on
strong cation-exchange (SCX) separation in the first
dimension and reversed phase (RP) separation in the
second dimension. Sophisticated set-ups using ternary
or quaternary gradient systems already exist to stepelute
peptides from the SCX column onto the RP column
followed by gradient elution and MS/MS analysis. These
methods are inherently complex and require highly skilled
and experienced specialists for successful development
and implementation.

Related Products

EASY-nLC
EASY-nLC II

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