Automated Acquisition of MALDI-ISD Spectra for the N- and C-terminal Sequence Determination of Intact Proteins

Technical Note

Expression Proteomics

TN-36

MALDI-TOF

2009

ultrafleXtreme BioTools

Anja Resemann, Detlev Suckau, Bruker Daltonik, Bremen Germany

Bruker Daltonics Technical Note TN-36

Abstract

For over 10 years, MALDI-Top-Down Sequencing
(MALDI-TDS) of intact proteins has proved particularly
useful for the determination of N- and C-terminal protein
sequences. This technology note describes a procedure
using the recently introduced 1,5-diaminonaphthalene
(DAN) matrix and the new ultrafleXtreme™ MALDI-TOF/
TOF that automates the entire analysis – from sample
preparation and data acquisition to N- and C-terminal
sequence assignments. 

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