Isotope-Coded Protein Labeling (ICPL) for Quantitative Proteomics Workflows Compatible with Protein Separation Strategies

Application Note

Expression Proteomics

MT-106

MALDI-TOF

2010

ultrafleXtreme, EASY-nLC, ProteinScape, PROTEINEER sp, SERVA ICPL Quadruplex PLUS Kit (#258968)

Anja Resemann, Waltraud Evers, Detlev Suckau; Bruker Daltonik GmbH, Bremen, Germany

Bruker Daltonics Application nOte MT-106

Abstract

The new Serva ICPL^TM Quadruplex PLUS (ICPL 4-plex)
protein quantification kit was used to establish instrument
and data analysis methods on the ultrafleXtreme MALDITOF/
TOF instrument. Resulting data were analyzed
using ProteinScape 2.1 that provide good quantification
results from ICPL 4-plex measurements. We analysed a
protein mixture that was labelled in 4 states with defined
quantities to validate the compatibility of the ICPL 4-plex
Kit with the bioinformatics suite ProteinScape 2.1, and the
MALDI-TOF/TOF. 

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ultrafleXtreme series

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