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MALDI Top-Down Sequencing: Calling N- and C-Terminal Protein Sequences with High Confidence and Speed
| Type: | Application |
Scientific Paper |
Expression Proteomics |
| Number: | Technology |
PMC2777352 |
MALDI-TOF |
| Year | Products |
2009 |
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| Author | |
Detlev Suckau and Anja Resemann Bruker Daltonics, Bremen, Germany Address correspondence to: Detlev Suckau, Bruker Daltonics, Fahrenheitstr. 4, D-28359, Bremen, Germany; E-mail: dsu@bdal.de |
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| Reference | |
J Biomol Tech. 2009 December; 20(5): 258–262. |
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Abstract |
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The ability to match Top-Down protein sequencing (TDS) results by MALDI-TOF to protein sequences by classical protein database searching was evaluated in this work. Resulting from these analyses were the protein identity, the simultaneous assignment of the N- and C-termini and protein sequences of up to 70 residues from either terminus. In combination with de novo sequencing using the MALDI-TDS data, even fusion proteins were assigned and the detailed sequence around the fusion site was elucidated. MALDI-TDS allowed to efficiently match protein sequences quickly and to validate recombinant protein structures—in particular, protein termini—on the level of undigested proteins.
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